United beat Real Betis 3-0 in a closed door friendly.
Rooney made an unexpected return from a hamstring injury, as Nemanja Vidic, Nani and Javier Hernandez also lined up in the match played at United's Carrington training ground.
The inclusion of Rooney comes as something of a surprise, as he was rated as doubtful for the start of the season after being ruled out for a month when he pulled up in training on United's tour of Thailand.
The four first-teamers were joined by a selection of youth-team players, and easily overcame a young Betis side.
Although the match took place in private, with no media access, Betis coach Pepe Mel let slip the result on Twitter, saying he was happy with his side's performance.
Betis continue their English tour with matches against Fulham, Everton and Hull City in the next week.
WHAT DOES ROONEY'S RETURN MEAN?
Wayne Rooney's swift recovery from injury can be seen in two, very different, ways.
An optimist would see it as a positive development, showing his continued ability to bounce back from injury, while reaffirming his commitment to United this season.
However, his appearance adds weight to the argument of those who suspected Rooney's departure from the Asian Tour had more to do with his possible transfer to Chelsea than a pulled hamstring.
United play the final game of their pre-season Tour away to AIK in Sweden on Tuesday, before facing Sevilla in Rio Ferdinand's testimonial on Friday.
But the most significant pointer as to Rooney's future comes next Sunday when United take on Wigan Athletic in the Community Shield at Wembley.
What is certain is that Rooney will rarely stray far from the headlines as the transfer window enters its final month.
