Using a 2.5 drive as your main system drive may actually hurt performance as 2.5 hdd usually are at 5400rpm. Their read write speed is usually slower than 3.5 hdd as 3.5 hdd are at 7200rpm
one option would be to go with WD VelociRaptor 2.5" drive (it is 10Krpm) but usually the reason to have 2.5 in desktop is to have silent pc.
once you place system on dedicated drive it will improve performance (without any stripes/raid0)
but as Entry Level PC said it will be better to get 120Gb ssd for system only (you don't need 256Gb as system won't take so much space and performance gains from ssd with bigger size won't be used by system purposes anyway)
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